HAMAMATSU
ORCA-Flash4.0 V3 Digital CMOS Camera C13440-20CU
We’ve advanced our camera technology, so you can advance your science.
Building on our extensive experience with high performance scientific cameras and advanced imaging applications, Hamamatsu introduces the new ORCA-Flash4.0 V3. This one camera expertly handles applications ranging from the acquisition of beautiful scientific images to experiments that demand detection, quantification and speed. With on-board FPGA processing enabling intelligent data reduction, highly refined in-camera, pixel-level calibrations, increased USB3.0 frame rates, purposeful and innovative triggering capabilities, patented lightsheet read out modes and individual camera noise characterization the ORCA-Flash4.0 V3 is the precision instrument for imaging.
- Effective no. of pixels: 2304 (H) × 2304 (V)
- Readout speed: 89.1 frames/s
- Readout noise: 0.7 electrons
- Dynamic range: 21 400:1
Features
- Calibrated for Quantitative Accuracy
- Flexibility for Customized Data Control
- Patented Tools for Advanced Imaging: Lightsheet Readout Mode
- Focus on the Relevant Data
- Powerful Triggering for Synchronization
Lightsheet Readout Mode [Patented]
“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
Utilizing selective plane illumination, Lightsheet microscopy is a fluorescence method that places a thin sheet of excitation light across only the focal plane being imaged. The sheet of light is swept in very precise synchronization to the rolling shutter of the camera. The scan height of the rolling shutter effectively becomes a slit, enabling less background signal and scattered light. By using this combination of illumination and camera readout, very fast acquisition of optically sectioned images is enabled. Additionally, there is significantly reduced photobleaching and phototoxicity to the sample as compared to many other methods.
Lightsheet microscopy is used for live cell imaging at scales that range from small groups of cells to whole organisms. The rapid increase in the use of this technique over the past several years has been remarkable.
PC Recommendations
With the introduction of the ORCA-Flash4.0, users are now able to stream 4 megapixel images to their computers 100 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Flash4.0.
Specifications
- Product number: C15440-20UP
- Imaging device: Scientific CMOS image sensor
- Effective number of pixels: 2304 (H) × 2304 (V)
- Pixel Size: 6.5 μm × 6.5 μm
- Effective Area: 14.976 mm (H) × 14.976 mm (V)
- Full well capacity: 15 000 electrons
- Readout speed: Fast scan, Full resolution, CoaXPress : 89.1 frames/s
Fast scan, Full resolution, USB 3.0 : 31.6 frames/s（16 bit）, 42.2 frames/s（12 bit） , 63.3 frames/s（8 bit）
Standard scan, Full resolution, CoaXPress : 23.2 frames/s
Standard scan, Full resolution, USB 3.0 : 23.2 frames/s
Ultra quiet scan, Full resolution, CoaXPress : 5.42 frames/s
Ultra quiet scan, Full resolution, USB 3.0 : 5.42 frames/s
- Readout noise: Fast scan : 1.6 electrons rms
Standard scan : 1.0 electrons rms
Ultra-quiet scan: 0.7 electrons rms
- Exposure times: Fast scan : 17 µs to 10 s
Standard scan : 65 µs to 10 s
Ultra quiet scan : 280 µs to 10 s
- Cooling method: Peltier cooling
- Cooling temperature: Forced-air cooled (Ambient temperature: +25 ˚C) : −8 ˚C
Water cooled (Water temperature: +25 ˚C) : −8 ˚C
Water cooled (Max cooling (Ambient temperature: +20 ˚C, water temperature: +20 ˚C)) : Less than −15 ˚C
- Dark current: 1.0 electrons/pixel/s (cooling temperature: -8 ˚C , Ambient temperature: +25 ˚C)
1.0 electrons/pixel/s (cooling temperature: -8 ˚C , Water temperature: +25 ˚C)
0.7 electrons/pixel/s (cooling temperature: Less than −15 ˚C , Ambient temperature: +20 ˚C, water temperature: +20 ˚C)
- Dynamic range: 21 400:1
- External trigger mode: Edge, Level, Sync readout, Start, Global reset edge, Global reset level, Programmable
- External trigger signal routing: SMA
- External trigger delay function: 0 μs to 10 s (1 μs step)
Trigger output Global exposure timing, Trigger ready, Low, High, 3 programmable timing outputs
- External signal output routing: SMA
- Interface: CoaXPress (Dual CXP-6) and USB 3.0 Super Speed
- A/D converter: 16 bit, 12 bit, 8 bit
- Lens mount: C-mount (standard), F-mount (C15440-20UP01)
- Power supply: AC 100 V to AC 240 V, 50 Hz/60 Hz
- Power consumption: Approx. 155 VA
- Ambient operating temperature: 0 °C to +40 °C
- Ambient storage temperature: -10 ℃ to +50 ℃
- Ambient operating humidity: 30 % to 80 % (With no condensation)
- Ambient storage humidity: 90 % or less (With no condensation)
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