Immersion media between the sample and the objective is required for high-resolution imaging. Manual addition of immersion media risks loss of data points or even microscope damage from user error; it is also tedious and inefficient. The Autoimmersion Module for ZEISS Axio Observer 7 widefield and confocal systems is your automated, easy-to-use solution for maintaining immersion media for water immersion objectives.
✓ Automated, hands-free application of water for immersion
✓ Reduced risk to your experiments and microscopes
✓ Design of complex experiments for unsupervised data collection
✓ Improved efficiency and throughput
Improve Your Efficiency & Throughput
Improve your throughput by up to 2.5-fold by designing experiments that collect data during non-working hours, such as overnight or over the weekend.
Application Examples
HEK KO PEX5 cells expressing eGFP with a photocaged peroxisomal targeting signal type 1 were reconstituted with the peroxisomal import receptor PEX5. A light induced conformational change of the photocage leads to exposure of the peroxisomal targeting signal. If the WT PEX5 is expressed, accumulation of the eGFP signal in the dotted peroxisomes can be monitored (top row). In case of the mutated PEX5 (bottom row), even after 18 hours, no peroxisomal import could be detected.
When working with living samples, you might not know where your event of interest may occur. To capture the uptake of nanoparticles by macrophages, many locations from a multi-well plate are acquired as well as multiple Z-planes over several hours at 37°C using re-immersion. The region shown above is a subset of the much larger dataset that was captured using automated imaging and shows the uptake of nanoparticles inside the cells (top row). The surface of the cells were also imaged to verify that the nanoparticles are inside the cells and not simple sitting on the cell surface (bottom row).
Red fluorescent small liposomes and different concentrations of Sar1p protein (partially labeled with Alexa Fluor 488) were mixed in a 96 multi-well plate and measured automatically over 15 hours. Krüger et al., Biophys. J. 2017. Sample courtesy of C. Haupt and K. Bacia, University of Halle, Germany
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