Combine the best for life science research in a single upright platform. Whether you are simply observing and recording or performing highly complex imaging experiments: It's easy to customize system components to meet the needs of your applications.
✓ Excellent optical performance for research-grade imaging
✓ Configuration flexibility to meet various application requirements
✓ Smart motorization to enable efficient workflows
With its freely accessible infinite space, additional components such as light sources and detectors can be added simply and rapidly as needed.
Managers for light and contrast ensure reproducible conditions. Auto Component Recognition ensures that objectives and filters are automatically detected.
Benefit from a wide range of objectives for both fixed and live cell imaging applications especially at high magnifications.
With the motorized DIC turret, you can automatically shift between different contrast methods to push either resolution or contrast.
For reliable reproducibility, objective-specific aperture adaptations can be saved and loaded again at any time.
The HE fluorescence filters provide high transmission for excitation and emission with up to 50 % shorter exposure times compared to conventional filters.
LED light sources combine constant color temperature independent of brightness, low heat radiation, and long service life.
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Whether you study cell compartmentalization, protein trafficking, cytoskeleton, cell division, cell death and apoptosis, stem cells and differentiation or many other topics in cell biology, fluorescence microscopy provides critical information for your research.
• How many fluorophores are required?
• How much resolution do you need?
• How sensitive are your samples?
• Do your experiments require high-content imaging?
Cortical neurons stained for DNA, microtubules and microtubule-associated proteins imaged with ZEISS Apotome 3. Courtesy of L. Behrendt, Leibniz-Institute on Aging – Fritz-Lipmann-Institut e.V. (FLI), Germany.
Cos7 cells imaged with LSM Plus, including the ZEISS NIR detector in channel mode. Sample courtesy of U. Ziegler and J. Doehner, University of Zurich, ZMB, Switzerland.
Lattice SIM² image of Cos-7 cells labeled with phalloidin Alexa 488. Maximum intensity projection of Z stack.
Eightfold symmetry of the nuclear pore complex in the frog kidney cell line, A6. Gp210 was labeled with Alexa Fluor 647. Widefield image (top row, left), SMLM image (top row, right) and zoomed in region (bottom row).
Multiplex imaging of non-small cell lung cancer (NSCLC) tissue stained with UltiMapper I/O PD-L1 kit. Nuclear counterstain (blue), CD8 (green), CD68 (orange), PD-L1 (red), panCytoKeratin(magenta) acquired with ZEISS Axioscan 7. Sample courtesy of Ultivue, Inc. Cambridge, Massachusetts, USA
Plunge-frozen adenocarcinoma cells grown on sapphire discs. All regions of interest are shown in context in ZEN Connect
When imaging large brain and tissue sections, you may find it difficult to set up a workflow that successfully collects your entire sample in high resolution. Or the length of time required to scan your entire sample or all your slides in the resolution you need is unachievable.
Colon tissue section from a patient with Crohn’s disease imaged using digital slide scanner. Courtesy of S. Seier Poulsen, University of Copenhagen, Denmark *
Non-small cell lung cancer (NSCLC) tissue section imaged using digital slide scanner. Fluorescence staining with UltiMapper I/O PD L1 kit. Sample courtesy of Ultivue, Inc., USA*
Non-small cell lung cancer (NSCLC) tissue section, picture detail Framed in the image (on the left side)
Mouse brain slice, captured using widefield fluorescence microscopy and tiling. Sample courtesy of D. Mi, School of Life Sciences, Tsinghua University, China
Overview image of mouse brain section collected using digital slide scanner
Insert A shows enlarged ROI collected using confocal microscopy with Airyscan super-resolution detector.
Insert B shows enlarged ROI collected using confocal microscopy with Airyscan super-resolution detector.
Mouse brain section, area 1 mm². Sample: courtesy of J. Lichtman, Harvard University, USA
Imaging living cells allows a better understanding of biological function through the study of cellular dynamics. From cell motility to cell-cell interactions, protein trafficking to cytoskeleton dynamics, cell cycle or cell death/apoptosis or many other topics in cell biology, live cell imaging may be critical to your research. In addition to balancing your needs for sensitivity, resolution and fluorophore requirements, you now have the added challenges of protecting your samples from phototoxicity with gentle imaging while balancing your need to acquire images at a faster speed. Perhaps you need options for photomanipulation. Your experiments may also require increased throughput, which can be very challenging with live samples.
HeLa cell culture with cytosolic eGFP. Proliferation imaged over 16 hours.
One of the most sensitive and fast solutions for live cell imaging is with widefield microscopes, such as the ZEISS Axio Observer inverted microscope. The entire field is imaged quickly using a high-speed camera. 3D data can be enhanced with deconvolution. Study the dynamics of multiple fluorophores quickly and with high resolution. The systems can be economical, straightforward and easy to use.
T-cells expressing LifeAct-GFP to visualize actin dynamics using lattice light sheet microscopy. Courtesy of M. Fritzsche, University of Oxford, United Kingdom.
ZEISS Lattice Lightsheet 7 uses light-sheet fluorescence microscopy for volumetric imaging of subcellular structures and dynamics over hours to days with the best protection from photo damage. The system is both extremely fast and remarkably gentle to living samples. Lattice Lightsheet 7 is built with integrated environmental controls to create the perfect setting for your samples.
Cell division of pig kidney epithelial cells (LLC-PK1) labeled for microtubules and histones, imaged with Airyscan SR in Multiplex mode.
The revolutionary design of the ZEISS LSM 9 family of confocal microscopes offer high sensitivity for crisp imaging and spectral flexibility for use of multiple fluorophores with overlapping excitation and emission spectra is possible with capabilities into the NIR as well as LSM Plus for improved resolution of spectral data.
Airyscan 2 can be added for super-resolution capabilities with Multiplex mode for higher speed to capture cellular dynamics.
Confocal microscopy is equipped for photomanipulation experiments such as photobleaching experiments (FRAP, FLIP, etc.), photoconversion and optogenetics. Molecular analyses such as FCS, RICS and FLIM are also available options.
Simultaneous imaging of the endoplasmic reticulum (Calreticulin-tdTomato, magenta) and microtubules (EMTB-3xGFP, green) in a Cos-7 cell reveals highly dynamic interaction of these organelles
ZEISS Elyra 7 enables you to combine super-resolution and high-dynamic imaging. With resolution down to 60 nm and speeds up to 255 fps, you can examine the dynamics of sub-cellular organelles with up to two fluorophores.
Human lung epithelial cell line A549 stained with MitoTracker Orange (mitochondria) and SIR-DNA (nuclei). Data collected with the fully automated Celldiscoverer 7. Sample courtesy of A.C. Hocke, Charité, Berlin, Germany
For high content, fully automated experiments, ZEISS Celldiscoverer 7 calibrates itself, detects and focuses on your samples with automatic optics adjustments. Fully integrated environmental controls create the perfect conditions for your samples for the duration of your experiment.
Celldiscoverer 7 easily combined with confocal microscope, giving you access to enhanced 3D resolution, high speed super-resolution imaging, photomanipulation and more. Acquire more data for robust analyses with this reliable, automated live cell imaging platform.
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