Apotome 3

ZEISS Apotome 3​

Optical Sectioning in Fluorescence Imaging for Your Widefield Microscope​

With structured illumination, removal of out-of-focus light becomes simple and efficient. ZEISS Apotome 3 calculates your optical section from a number of images acquired with different grid positions. Get high-contrast images, even from thicker specimens, while your system remains just as easy to operate as always.

• Brilliant optical sections​
• Based on peer-reviewed algorithms
• More structural information
• Free choice of light source and dyes​

VIEW SPECIFICATIONS AND APPLICATIONS

Brilliant Optical Sections​

Even From Thick Specimens

• Apotome 3 significantly increases the axial resolution compared to conventional fluorescence microscopy: You obtain optical sections that allow 3D rendering, even from thick specimens. Three grids of different geometries give you the best resolution for each objective. You can focus on your experiment as the ideal grid is automatically selected, always resulting in high-contrast optical sections.

Peer-reviewed Algorithms

Linear Approaches for True Optical Sections

• Software-based solutions require either prior knowledge of the​ sample (AI based methods) or rely on complex algorithms that​ have not been peer-reviewed. Users must trust that these​ black-box solutions only produce structures that are real. ZEISS Apotome 3 uses linear approaches and well documented algorithms, allowing you to calculate true, reliable optical sections.

More Structural Information

Compare Widefield, Optical Sectioning, and Deconvolution

• Improve your images even more by deconvolution, using an algorithm for structured illumination. While retaining all raw data, the system allows you to switch between widefield, optical section and deconvolved images for maximum flexibility and best comparability. The robust, easy to use deconvolution algorithms improve both lateral and axial resolution. Improved contrast and noise suppression let you better recognize the structures of the examined object.

Free Choice of Light Source and Dyes

It’s Your Decision, Not the Technology’s

• Your experiments often evolve in complexity and requirements. That’s why you need adaptable equipment. Use Apotome 3 with metal halide lamps, economic white light LEDs, or the gentle, multi-color Colibri illumination system. Whether you work with DAPI, Alexa488, Rhodamin, Cy5, or with vital dyes such as GFP or mCherry – Apotome 3 adapts to your fluorophores and light source, creating the sharp and brilliant images you expect.

DOWNLOADS

• 3D Imaging Systems ↴
  Your Guide to the Widest Selection of Optical Sectioning, Electron Microscopy and X-ray Microscopy Techniques.
• Zeiss Apotome 3 ↴
  Optical sectioning in fluorescence imaging for your widefield microscope.
• ZEISS Apotome 3 - Flyer ↴
  Hardware-based, Quantitative Optical Sectioning with Well Documented Algorithms.

Warranty

• 1 year manufacturer warranty.

Returns

• 20% restocking fee.

Disclaimer: Please note that we are an authorized dealer of Carl Zeiss products in New York State. Our services are exclusively available to clients within the state of New York. If you are not located in New York State and are interested in our products, please don't hesitate to get in touch with us for further information and assistance.