HAMAMATSU
ORCA-Fusion Digital CMOS Camera C14440-20UP
The ORCA-Fusion, built from the sensor up, balances the complex nuances of camera features to provide beautiful images and robust data at all lights levels, but especially in tough low-light conditions. The exceptionally low and highly uniform read noise of the ORCA-Fusion means that when the sample emits even just a handful of photons, either by default or by experimental design, they are not lost in the noise, but detected and reliably quantified. After all, when you want to hear a whisper it’s best to be in a quiet place.
- Effective no. of pixels: 2304(H)×2304(V)
- Readout speed: 89.1 frames/s
- Readout noise: 0.7 electrons
- Dynamic range: 21 400 : 1
Features
- High resolution : 2304 x 2304 (5.3 Megapixels)
- High speed : 100 frames/s (at 2304 X 2048 ROI)
- Dynamic range : 21,400:1
- Pixel size : 6.5 μm X 6.5 μm
- Read noise : 0.7 electrons, rms (Ultra quiet Scan)
- Prnu : 0.06 % (@ 7500 electrons)
- DSNU : 0.06 electrons
- Peak QE : 80 %
- Patented Tools for Advanced
- Imaging: Lightsheet Readout Mode
Lightsheet Readout Mode [Patented]
“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
Utilizing selective plane illumination, Lightsheet microscopy is a fluorescence method that places a thin sheet of excitation light across only the focal plane being imaged. The sheet of light is swept in very precise synchronization to the rolling shutter of the camera. The scan height of the rolling shutter effectively becomes a slit, enabling less background signal and scattered light. By using this combination of illumination and camera readout, very fast acquisition of optically sectioned images is enabled. Additionally, there is significantly reduced photobleaching and phototoxicity to the sample as compared to many other methods.
Lightsheet microscopy is used for live cell imaging at scales that range from small groups of cells to whole organisms. The rapid increase in the use of this technique over the past several years has been remarkable.
PC Recommendations
With the introduction of the ORCA-Fusion, users are now able to stream 5 megapixel images to their computers 89 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Fusion.
Specifications
- Type number: C14440-20UP
- Imaging device: sCMOS
- Effective no. of pixels: 2304(H)×2304(V)
- Cell size: 6.5 μm(H)×6.5 μm(V)
- Effective area: 14.976 mm (H)×14.976 mm (V)
- Full well capacity: 15 000 electrons
- Readout speed: Fast scan: 11.22 ms (89.1 frames/s with CoaXPress or 31.6 frames/s with USB 3.0) (2304 x 2304)
Standard scan: 42.99 ms (23.2 frames/s with CoaXPress or USB 3.0) (2304 x 2304)
Ultra quiet scan: 184.4 ms (5.4 frames/s with CoaXPress or USB 3.0) (2304 x 230
- Readout noise: Fast scan：1.4 electrons rms
Standard scan：1.0 electrons rms
Ultra quiet scan：0.7 electrons rms
- Exposure time: Fast scan: 17 μs to 10 s (4.87 μs step)
Standard scan: 65 μs to 10 s (18.65 μs step)
Ultra quiet scan: 280 μs| 10 s (80.00 μs step)
- Cooling method: Peltier cooling
Cooling temperature Forced-air cooled: -5 ˚C (Ambient temperature: +25 ˚C)
Water cooled: -5 ˚C (Water temperature: +25 ˚C)
Water cooled (Max cooling): Less than -15 ˚C
- Dark current: Cooling temperature(-5 ˚C): 0.5 electrons/pixel/s
Cooling temperature(-15 ˚C): 0.2 electrons/pixel/s
- Dynamic range: 21 400 : 1
- External trigger mode: Edge, Level, Sync readout, Start, Global reset edge, Global reset level
- External trigger signal routing: SMA
- Trigger delay function: 0 s to 10 s in 1 μs steps
- Trigger output: 3 programmable timing, Global exposure timing, trigger ready, low, high
- External signal output routing: SMA
- Interface: CoaXPress (Dual CXP-6) and USB 3.0 *6
- A/D converter: 16 bit / 12 bit / 8 bit
- Lens mount: C-mount
- Power supply: Approx. 150 VA
- Ambient operating temperature: 0 ˚C to + 40 ˚C
- Ambient operating humidity: 30 % to 80 %, with no condensation
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