HAMAMATSU
ORCA-Fusion BT Digital CMOS Camera C15440-20UP
See what you’ve been missing
The ORCA-Fusion BT camera is the pinnacle of scientific CMOS (sCMOS) performance. The specifications are without compromise: ultra-low readout noise, CCD-like uniformity, fast frame rates and back-thinned enabled high QE. But the beauty of the ORCA-Fusion BT is what this combination of exceptional photon detection and collection can do for you. See the dimmest whisper of signal, acquire visually stunning, high S/N images from the fewest photons, capture previously unresolved temporal events and perform computational methods with confidence. The ORCA-Fusion BT is the camera that makes the difficult experiments easy and previously impossible experiments possible.
- Effective no. of pixels: 2304 (H) × 2304 (V)
- Readout speed: 89.1 frames/s
- Readout noise: 0.7 electrons
- Dynamic range: 21 400:1
ORCA-Fusion BT Advantages Versus Other BT CMOS Cameras
Exclusive industry leading BT sCMOS performance with trusted Hamamatsu quality and reliability
- Extremely low readout noise
- High QE, across a wide range of wavelengths
- CCD-like uniformity; minimal high readout noise pixels
- High dynamic range
- 3 readout speeds
- Lightsheet readout mode
- 5.3 Megapixels
- Both USB 3.0 and high speed
- CoaXPress interfaces
ORCA-Fusion BT Advantages Versus EM-CCD Cameras
A practical and functional replacement for expensive EM-CCDs with uncompromising sensitivity, speed and versatility
- Resolution
- Binning versatility
- No amplification or excess noise
- High dynamic range
- Large field of view
Applications
- Wide-field fluorescence microscopy
- Time-lapse imaging
- Spinning disk confocal microscopy
- Lightsheet microscopy
- Super resolution precision localization
- Genetically encoded voltage imaging
- Optogenetics
- Single molecule tracking
- Computational imaging
- Quantum computing
Lightsheet Readout Mode [Patented]
“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
Utilizing selective plane illumination, Lightsheet microscopy is a fluorescence method that places a thin sheet of excitation light across only the focal plane being imaged. The sheet of light is swept in very precise synchronization to the rolling shutter of the camera. The scan height of the rolling shutter effectively becomes a slit, enabling less background signal and scattered light. By using this combination of illumination and camera readout, very fast acquisition of optically sectioned images is enabled. Additionally, there is significantly reduced photobleaching and phototoxicity to the sample as compared to many other methods.
Lightsheet microscopy is used for live cell imaging at scales that range from small groups of cells to whole organisms. The rapid increase in the use of this technique over the past several years has been remarkable.
PC Recommendations
With the introduction of the ORCA-Fusion BT, users are now able to stream 5 megapixel images to their computers 89 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Fusion BT.
Specifications
- Product number: C15440-20UP
- Imaging device: Scientific CMOS image sensor
- Effective number of pixels: 2304 (H) × 2304 (V)
- Pixel Size: 6.5 μm × 6.5 μm
- Effective Area: 14.976 mm (H) × 14.976 mm (V)
- Full well capacity: 15 000 electrons
- Readout speed: Fast scan, Full resolution, CoaXPress : 89.1 frames/s
Fast scan, Full resolution, USB 3.0 : 31.6 frames/s（16 bit）, 42.2 frames/s（12 bit） , 63.3 frames/s（8 bit）
Standard scan, Full resolution, CoaXPress : 23.2 frames/s
Standard scan, Full resolution, USB 3.0 : 23.2 frames/s
Ultra quiet scan, Full resolution, CoaXPress : 5.42 frames/s
Ultra quiet scan, Full resolution, USB 3.0 : 5.42 frames/s
- Readout noise: Fast scan : 1.6 electrons rms
Standard scan : 1.0 electrons rms
Ultra-quiet scan: 0.7 electrons rms
- Exposure times: Fast scan : 17 µs to 10 s
Standard scan : 65 µs to 10 s
Ultra quiet scan : 280 µs to 10 s
- Cooling method: Peltier cooling
- Cooling temperature: Forced-air cooled (Ambient temperature: +25 ˚C) : −8 ˚C
Water cooled (Water temperature: +25 ˚C) : −8 ˚C
Water cooled (Max cooling (Ambient temperature: +20 ˚C, water temperature: +20 ˚C)) : Less than −15 ˚C
- Dark current: 1.0 electrons/pixel/s (cooling temperature: -8 ˚C , Ambient temperature: +25 ˚C)
1.0 electrons/pixel/s (cooling temperature: -8 ˚C , Water temperature: +25 ˚C)
0.7 electrons/pixel/s (cooling temperature: Less than −15 ˚C , Ambient temperature: +20 ˚C, water temperature: +20 ˚C)
- Dynamic range: 21 400:1
- External trigger mode: Edge, Level, Sync readout, Start, Global reset edge, Global reset level, Programmable
- External trigger signal routing: SMA
- External trigger delay function: 0 μs to 10 s (1 μs step)
Trigger output Global exposure timing, Trigger ready, Low, High, 3 programmable timing outputs
- External signal output routing: SMA
- Interface: CoaXPress (Dual CXP-6) and USB 3.0 Super Speed
- A/D converter: 16 bit, 12 bit, 8 bit
- Lens mount: C-mount (standard), F-mount (C15440-20UP01)
- Power supply: AC 100 V to AC 240 V, 50 Hz/60 Hz
- Power consumption: Approx. 155 VA
- Ambient operating temperature: 0 °C to +40 °C
- Ambient storage temperature: -10 ℃ to +50 ℃
- Ambient operating humidity: 30 % to 80 % (With no condensation)
- Ambient storage humidity: 90 % or less (With no condensation)
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